[ Sr2+] Stimulation of α-amylase and RNAse in Wheat Aleurone Layer
نویسندگان
چکیده
منابع مشابه
In Vitro Synthesis and Processing of Wheat alpha-Amylase : TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT GERM AND XENOPUS LAEVIS OOCYTE SYSTEMS.
Wheat (Triticum aestivum) RNA was used to program synthesis of the alpha-amylase protein by Xenopus laevis oocytes. A 41,500-dalton protein was made which was identified as alpha-amylase by immunoprecipitation with rabbit anti-alpha-amylase antiserum raised against the purified wheat protein and by its co-migration with authentic alpha-amylase on sodium dodecyl sulfate polyacrylamide gels. Synt...
متن کاملOsmotic Regulation of Α-amylase Synthesis and Polyribosome Formation in Aleurone Cells of Barley
Water stress inhibits the gibberellic acid (GA(3))-induced synthesis of alpha-amylase in aleurone layers of barley (Hordeum vulgare L.). Electron microscope evidence indicates that the effect of water stress induced by 0.6 M solutions of polyethylene glycol (PEG) is to reduce the binding of ribosomes to the endoplasmic reticulum. This was confirmed by sucrose density gradient centrifugation of ...
متن کاملSpatio-temporal appearance of α-amylase and limit dextrinase in barley aleurone layer in response to gibberellic acid, abscisic acid and salicylic acid.
BACKGROUND Cereal seed germination involves mobilization of storage reserves in the starchy endosperm to support seedling growth. In response to gibberellin produced by the embryo the aleurone layer synthesizes hydrolases that are secreted to the endosperm for degradation of storage products. In this study analysis of intracellular protein accumulation and release from barley aleurone layers is...
متن کاملIMMUNOHISTOCHEMTCAL LOCALIZATION OF a-AMYLASE IN BARLEY ALEURONE CELLS
a-Amylase was localized in aleurone cells of barley using immunohistochemical methods. Anti-a-amylase antibody was produced by rabbits immunized with enzyme purified from malt diastase and Himalaya variety barley seeds. Immunoelectrophoresis showed that the antibodies to both antigens were immunologically similar, therefore, they were used interchangeably in the localization of a-amylase. Fluor...
متن کاملInhibitory effect of aqueous extract of Ocimum Gratissimum on digestive enzymes of carbohydrates, α-Amylase and α-Glucosidase in-vitro
For a long period, ethno medicinal plants have been a valuable source of natural products for maintaining human health, especially in the last decade, with more intensive studies for natural therapies. The use of ethno medicinal plant for pharmaceutical purposes has gradually increased in Iran. Gundelia tournefortii has been used as an antibacterial, anti-fungal, antipyretic, anti-inflammatory,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Korean Journal of Environmental Agriculture
سال: 2003
ISSN: 1225-3537
DOI: 10.5338/kjea.2003.22.4.290